Journal: Oncotarget

Article Title: Metabolic reprogramming of glioblastoma cells by L-asparaginase sensitizes for apoptosis in vitro and in vivo

doi: 10.18632/oncotarget.9257

Figure Lengend Snippet: A. , SF188 and LN299 glioblastoma cells were treated for 72h with L-asparaginase and ABT263 as indicated prior to performing MTT-assays. Normalized isobolograms were calculated using the CompuSyn software. The connecting line represents additivity. Data points located below the line indicate a synergistic drug-drug interaction and data points above the line indicate an antagonistic drug-drug interaction. B. , Representative flow plots of SF188 (Asp 0.5IU/ml, ABT263 1μM, 24h), LN229 (Asp 2IU/ml, ABT263 0.5μM, 24h), U251 (Asp 2IU/ml, ABT263 1.5μM, 24h) and T98G (Asp 1IU/ml, ABT263 1μM, 48h) glioblastoma cells subjected to treatment with L-asparaginase, ABT263 or the combination prior to staining for annexin V/propidium iodide and flow cytometric analysis. C. , SF188 and LN229 glioblastoma cells were treated with L-asparaginase and/or ABT263 as indicated. Western blot analysis was performed for caspase 9 (CP9), cleaved caspase 3 (cCP3), cleaved PARP (cPARP), Mcl-1, Usp9X and Noxa. Actin expression was determined to confirm equal protein loading. CF = cleaved fragment; arrow head represents specific band for Mcl-1. D. , Representative microphotographs of LN229 glioblastoma cells treated with 10 IU/ml L-asparaginase, 2 μM ABT263 or the combination for 48h. Magnification, x40; scale bar, 100 μm.

Article Snippet: The CompuSyn software (ComboSyn, Inc., Paramus, NJ - www.combosyn.com last accessed 06/01/15) was used for the drug combination analysis including the calculation of the combination index (CI) and isobologram as described before [ ].

Techniques: Software, Staining, Western Blot, Expressing

Journal: Oncotarget

Article Title: Metabolic reprogramming of glioblastoma cells by L-asparaginase sensitizes for apoptosis in vitro and in vivo

doi: 10.18632/oncotarget.9257

Figure Lengend Snippet: A. , SF188 and LN299 glioblastoma cells were treated for 72h with L-asparaginase and TRAIL as indicated prior to performing MTT-assays. Normalized isobolograms were calculated using the CompuSyn software. The connecting line represents additivity. Data points located below the line indicate a synergistic drug-drug interaction and data points above the line indicate an antagonistic drug-drug interaction. B. , Representative flow plots of SF188 (Asp 0.5IU/ml, TRAIL 25 ng/ml), LN229 (Asp IU/ml, TRAIL 100 ng/ml) and T98G (Asp 0.75IU/ml, TRAIL 4ng/ml) glioblastoma cells subjected to treatment with L-asparaginase, TRAIL or the combination for 24h prior to staining for annexin V/propidium iodide and flow cytometric analysis. C. , SF188 and LN229 glioblastoma cells were treated with L-asparaginase and/or TRAIL as indicated. Western blot analysis was performed for caspase8 (CP8), cleaved caspase 3 (cCP3), cleaved PARP (cPARP), Mcl-1, Usp9X and Noxa. Actin expression was determined to confirm equal protein loading. D. , Representative flow plots of T98G glioblastoma cells treated with increasing concentrations of L-asparaginase prior to staining for JC-1 and flow cytometric analysis. E, Representative microphotographs of LN229 glioblastoma cells treated with 2 IU/ml L-asparaginase, 250 ng/ml TRAIL or the combination for 48h. Magnification, x40; scale bar, 100 μm.

Article Snippet: The CompuSyn software (ComboSyn, Inc., Paramus, NJ - www.combosyn.com last accessed 06/01/15) was used for the drug combination analysis including the calculation of the combination index (CI) and isobologram as described before [ ].

Techniques: Software, Staining, Western Blot, Expressing